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KMID : 0350519930460010391
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Journal of Catholic Medical College
1993 Volume.46 No. 1 p.391 ~ p.399
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Comparison of Coulter Stack-S and Mamual Method with FCM-reference Method in Leukocyte Differential Counts
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Abstract
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To determine the accuracy of methods for measuring the leukocyte differential count the results obtained by three different counters such as flow cytometry(FCM), Coultor Stack-S(STKS), and manual count were analysed in normal, leukocytosis, and leukopenia roups, respectively.
The FCM analysis by using dual staining with monoclonal antibodies Mo2-RD1/KC56-FITC was used as the reference method. MsIgG1-FITC was used as a negative control also.
In order to establish the improved accuracy of the STKS, when compared with manual technique, linear regression analysis and pairad t-test were done to evaluate the correlation and mean percentage values between reference method and manual
methods as well as between reference method and STKS.
@ES The results were as follows :@EN 1. In the normal group reference method with STKS, as well as reference method with manual method showed strong positive correlations among granulocyte counts and lymphocyte counts and lymphocyte counts, how ever, the former showed stronger correlation.
There was a moderate positie correlation among monocyte counts, as measured with FCM and with STKS.
2. In the leukocytosis group, strong positive correlations were noted among granulocyte counts and lymphocyte counts, when measured by reference method compared with either STKS or manual method, and reference method compared to the STKS revealed
stronger correlation than that compared to manual method.
There were good correlations among monocyte counts, as measured by reference method and by each of the remaining two.
3. In the leukopenia group, all the results obtained by reference method comapared with STKS and by reference method compared with manual method were correlated very well in granulocyte, lymphocyte and monocyte counts.
4. Mean values of granulocyte and of lymphocyte, as measurd with FCM and with STKS, showed significant differences in all three groups, however, those of monocyte were significantiy different only in normal and leukopenia groups.
Mean values of granulocyte and monocyte counts, as measured by FCM and by manual method, revaled significant differences in normal group those of monocyte count significant difference in leukocytosis group, and those of three part leukocyte differential
counts no significant differences in leukopenia group.
In conclusion, STKS was noted to be an acceptable hematology analyzer ror leukocyte differential counts and it can replace the manual method.
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